Hyperglycemia in diabetes melilitus has been associated with increased formation of reactive oxygen species and oxidative damage to tissue compounds The main aim of this study was to evaluate the antioxidant effect of ascorbic acid in streptozotocin induced diabetic rats. Ascorbic acid is a naturally occurring organic compound with antioxidant properties. All albino wistar rats were divided into four groups and each group contained six animals. Group I (Normal control) received vehicle, group II (Diabetic control) received STZ (50 mg/kg i.p.). Group III and IV received STZ (50 mg/kg i.p.) along with ascorbic acid 500 mg/kg and 1000 mg/kg respectively. All the groups were treated for fifteen days. On 15th day the blood was collected by retro orbital sinus punctured method and centrifuged at 3000 rpm for 10 min to separate the serum which was used for the determination of antioxidant enzymes, lipid peroxidation and uric acid. The result shows the reduced antioxidant enzyme level such as SOD, CAT and peroxidase in diabetic rats were significantly increased by ascorbic acid. The lipid peroxidation and uric acid level in serum from diabetic rats were significantly decreased by ascorbic acid. It was concluded that the administration of ascorbic acid in diabetic rats can exert beneficial effects of anti oxidative defence system against that imposed by diabetes mellitus.
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